Effect of fentanyl on HIV expression in peripheral blood mononuclear cells.

ABSTRACT:

Introduction

Illicit drug use, particularly the synthetic opioid fentanyl, presents a significant global health challenge. Previous studies have shown that fentanyl enhances viral replication; yet, the mechanisms by which it affects HIV pathogenesis remain unclear. This study investigated the impact of fentanyl on HIV replication in CD4⁺ T lymphocytes.

Methods

CD4⁺ T lymphocytes from HIV-negative donors were activated, infected with HIV_NL4-3, and treated with fentanyl. HIV proviral DNA and p24 antigen expression were quantified using real-time PCR and ELISA, respectively. Single-cell RNA libraries were analyzed to identify differentially expressed genes.

Results

Results indicated that fentanyl treatment increased HIV p24 expression and

SUBMITTER: Krishnan JM 

PROVIDER: S-EPMC11461324 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature