In vivo thrombin activity in the diatom Phaeodactylum tricornutum: biotechnological insights.

ABSTRACT: Diatoms are responsible for 20% of global carbon dioxide fixation and have significant potential in various biotechnological and industrial applications. Recently, the pennate diatom Phaeodactylum tricornutum has emerged as a prominent platform organism for metabolic engineering and synthetic biology. The availability of its genome sequence has facilitated the development of new bioengineering tools. In this study, we used in silico analyses to identify sequences potentially encoding thrombin-like proteins, which are involved in recognizing and cleaving the thrombin sequence LVPRGS in P. tricornutum. Protein structure prediction and docking studies indicated a similar active site and ligand positioning compared to characterized human and bovine thrombin. The evidence and efficiency of the

SUBMITTER: Messaabi A 

PROVIDER: S-EPMC11461642 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature