Elucidation of the pluripotent potential of bovine embryonic lineages facilitates the establishment of formative stem cell lines.

ABSTRACT: The establishment of epiblast-derived pluripotent stem cells (PSCs) from cattle, which are important domestic animals that provide humans with milk and meat while also serving as bioreactors for producing valuable proteins, poses a challenge due to the unclear molecular signaling required for embryonic epiblast development and maintenance of PSC self-renewal. Here, we selected six key stages of bovine embryo development (E5, E6, E7, E10, E12, and E14) to track changes in pluripotency and the dependence on signaling pathways via modified single-cell transcription sequencing technology. The remarkable similarity of the gene expression patterns between cattle and pigs during embryonic lineage development contributed to the successful establishment of bovine epiblast stem cells (bEpiSCs) using

SUBMITTER: Zhi M 

PROVIDER: S-EPMC11461730 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature