Unmasking AlphaFold to integrate experiments and predictions in multimeric complexes.

ABSTRACT: Since the release of AlphaFold, researchers have actively refined its predictions and attempted to integrate it into existing pipelines for determining protein structures. These efforts have introduced a number of functionalities and optimisations at the latest Critical Assessment of protein Structure Prediction edition (CASP15), resulting in a marked improvement in the prediction of multimeric protein structures. However, AlphaFold's capability of predicting large protein complexes is still limited and integrating experimental data in the prediction pipeline is not straightforward. In this study, we introduce AF_unmasked to overcome these limitations. Our results demonstrate that AF_unmasked can integrate experimental information to build larger or hard to predict protein assemblies with

SUBMITTER: Mirabello C 

PROVIDER: S-EPMC11461844 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature