Structural and functional insights into the T-even type bacteriophage topoisomerase II.

ABSTRACT: T-even type bacteriophages are virulent phages commonly used as model organisms, playing a crucial role in understanding various biological processes. One such process involves the regulation of DNA topology during phage replication upon host infection, governed by type IIA DNA topoisomerases. In spite of various studies on prokaryotic and eukaryotic counterparts, viral topoisomerase II remains insufficiently understood, especially the unique domain composition of T4 phage. In this study, we determine the cryo-EM structures of topoisomerase II from T4 and T6 phages, including full-length structures of both apo and DNA-binding states which have never been determined before. Together with other conformational states, these structures provide an explicit blueprint of mechanisms of phage topoi

SUBMITTER: Xin Y 

PROVIDER: S-EPMC11461880 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature