Decellularized liver scaffolds for constructing drug-metabolically functional <i>ex vivo</i> human liver models.

ABSTRACT: The creation of ex vivo human liver models has long been a critical objective in academic, clinical, and pharmaceutical research, particularly for drug development, where accurate evaluation of hepatic metabolic dynamics is crucial. We have developed a bioengineered, perfused, organ-level human liver model that accurately replicates key liver functions, including metabolic activities, and protein synthesis, thus addressing some of the limitations associated with traditional liver monolayers, organoids, and matrix-embedded liver cells. Our approach utilizes liver-specific biomatrix scaffolds, prepared using an innovative protocol and fortified with matrix components that facilitate cellular interactions. These scaffolds, when seeded with human fetal liver cells or co-seeded with live

SUBMITTER: Liu J 

PROVIDER: S-EPMC11462156 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature