Cellular and molecular determinants of bacterial burden in leprosy granulomas revealed by single-cell multimodal omics.

ABSTRACT:

Background

Which cell populations that determine the fate of bacteria in infectious granulomas remain unclear. Leprosy, a granulomatous disease with a strong genetic predisposition, caused by Mycobacterium leprae infection, exhibits distinct sub-types with varying bacterial load and is considered an outstanding disease model for studying host-pathogen interactions.

Methods

We performed single-cell RNA and immune repertoire sequencing on 11 healthy controls and 20 patients with leprosy, and integrated single-cell data with genome-wide genetic data on leprosy. Multiplex immunohistochemistry, and in vitro and in vivo infection experiments were conducted to confirm the multimodal omics findings.

Findings

Lepromatous leprosy (L-LEP) granulomas with high bacterial burden we

SUBMITTER: Mi Z 

PROVIDER: S-EPMC11462173 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature