DNA demethylation triggers cell free DNA release in colorectal cancer cells.

ABSTRACT:

Background

Liquid biopsy based on cell-free DNA (cfDNA) analysis holds significant promise as a minimally invasive approach for the diagnosis, genotyping, and monitoring of solid malignancies. Human tumors release cfDNA in the bloodstream through a combination of events, including cell death, active and passive release. However, the precise mechanisms leading to cfDNA shedding remain to be characterized. Addressing this question in patients is confounded by several factors, such as tumor burden extent, anatomical and vasculature barriers, and release of nucleic acids from normal cells. In this work, we exploited cancer models to dissect basic mechanisms of DNA release.

Methods

We measured cell loss ratio, doubling time, and cfDNA release in the supernatant of a colorectal ca

SUBMITTER: Pessei V 

PROVIDER: S-EPMC11462661 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature