Discovering cancer stem-like molecule, nuclear factor I X, using spatial transcriptome in gastric cancer.

ABSTRACT: Gastric cancer (GC) is characterized by significant intratumoral heterogeneity, and stem cells are promising therapeutic targets. Despite advancements in spatial transcriptome analyses, unexplored targets for addressing cancer stemness remain unknown. This study aimed to identify Nuclear Factor IX (NFIX) as a critical regulator of cancer stemness in GC and evaluate its clinicopathological significance and function. Spatial transcriptome analysis of GC was conducted. The correlation between NFIX expression, clinicopathological factors, and prognosis was assessed using immunostaining in 127 GC cases. Functional analyses of cancer cell lines validated these findings. Spatial transcriptome analysis stratified GC tissues based on genetic profiles, identified CSC-like cells, and further refined

SUBMITTER: Ishikawa A 

PROVIDER: S-EPMC11462935 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature