Signal sequences target enzymes and structural proteins to bacterial microcompartments and are critical for microcompartment formation.

ABSTRACT: Spatial organization of pathway enzymes has emerged as a promising tool to address several challenges in metabolic engineering, such as flux imbalances and off-target product formation. Bacterial microcompartments (MCPs) are a spatial organization strategy used natively by many bacteria to encapsulate metabolic pathways that produce toxic, volatile intermediates. Several recent studies have focused on engineering MCPs to encapsulate heterologous pathways of interest, but how this engineering affects MCP assembly and function is poorly understood. In this study, we investigated the role of signal sequences, short domains that target proteins to the MCP core, in the assembly of 1,2-propanediol utilization (Pdu) MCPs. We characterized two novel Pdu signal sequences on the structural proteins

SUBMITTER: Johnson ER 

PROVIDER: S-EPMC11463388 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature