A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA.

ABSTRACT: Malonyl-CoA is the essential building block of fatty acids and regulates cell function through protein malonylation and allosteric regulation of signaling networks. Accordingly, the production and use of malonyl-CoA is finely tuned by the cellular energy status. Most studies of malonyl-CoA dynamics rely on bulk approaches that take only a snapshot of the average metabolic state of a population of cells, missing out on dynamic changes in malonyl-CoA and fatty acid biosynthesis that could be occurring within a single cell. To overcome this limitation, we have developed a genetically encoded fluorescent protein-based biosensor for malonyl-CoA that can be used to capture malonyl-CoA dynamics in single cells. This biosensor, termed Malibu (malonyl-CoA intracellular biosenso

SUBMITTER: Ranzau BL 

PROVIDER: S-EPMC11463626 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature