Single-nucleus multiomics reveals the gene-regulatory networks underlying sex determination of murine primordial germ cells.

ABSTRACT: Accurate specification of female and male germ cells during embryonic development is critical for sexual reproduction. Primordial germ cells (PGCs) are the bipotential precursors of mature gametes that commit to an oogenic or spermatogenic fate in response to sex-determining cues from the fetal gonad. The critical processes required for PGCs to integrate and respond to signals from the somatic environment in gonads are not understood. In this study, we developed the first single-nucleus multiomics map of chromatin accessibility and gene expression during murine PGC development in both XX and XY embryos. Profiling of cell-type specific transcriptomes and regions of open chromatin from the same cell captured the molecular signatures and gene networks underlying PGC sex determination. Joint R

SUBMITTER: Alexander AK 

PROVIDER: S-EPMC11463670 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature