Hederagenin regulates the migration and invasion of hepatocellular carcinoma cells through FOXO signaling pathway.

ABSTRACT:

Objective

This study aimed to elucidate the effects of Hederagenin (HG) on hepatocellular carcinoma (HCC) and explore its potential molecular mechanisms.

Materials and methods

Virtual screening was employed to identify potential targets within core pathways of liver cancer and to analyze the possible mechanisms of HG. CCK-8 assays were used to assess the viability of HCC cells, while Hoechst 33342/PI staining was utilized to evaluate apoptosis. The migration and invasion abilities of HCC cells were examined using Transwell and scratch assays, and single-cell cloning ability was assessed via colony formation assays. Subsequent qRT-PCR was conducted to determine the mRNA expression levels of FOXO1 and FOXO6 following HG treatment. Western blot (WB) analysis was employed to mea

SUBMITTER: Bao S 

PROVIDER: S-EPMC11463763 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature