Tissue-specific and endogenous protein labeling with split fluorescent proteins.

ABSTRACT: The ability to label proteins by fusion with genetically encoded fluorescent proteins is a powerful tool for understanding dynamic biological processes. However, current approaches for expressing fluorescent protein fusions possess drawbacks, especially at the whole organism level. Expression by transgenesis risks potential overexpression artifacts while fluorescent protein insertion at endogenous loci is technically difficult and, more importantly, does not allow for tissue-specific study of broadly expressed proteins. To overcome these limitations, we have adopted the split fluorescent protein system mNeonGreen2_1-10/11 (split-mNG2) to achieve tissue-specific and endogenous protein labeling in zebrafish. In our approach, mNG2_1-10 is expressed under a tissue-specific

SUBMITTER: Ligunas GD 

PROVIDER: S-EPMC11463824 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature