Novel RNA biomarkers improve discrimination of children with tuberculosis disease from those with non-TB pneumonia after <i>in vitro</i> stimulation.

ABSTRACT: The diagnosis of pediatric tuberculosis (TB) poses a challenge for clinical teams worldwide. TB-mediated changes in the expression of host genes in the peripheral blood can serve as diagnostic biomarkers and can provide better insights into the host immune mechanisms of childhood TB. Peripheral blood mononuclear cells (PBMCs) from children (n=102) with microbiologically confirmed TB disease, TB infection (TBI), pneumonia, and healthy controls (HC) were stimulated with either the Purified Protein Derivative (PPD) or the Early Secretory Antigen 6kDa-Culture Filtrate Protein 10 (ESAT6-CFP10) complex of Mycobacterium tuberculosis (Mtb). RNA was extracted and quantified using gene expression microarrays. Differential expression analysis was performed comparing microbiologically co

SUBMITTER: Vito O 

PROVIDER: S-EPMC11464340 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature