Metabolomic profiling reveals the step-wise alteration of bile acid metabolism in patients with diabetic kidney disease.

ABSTRACT:

Background

Diabetic kidney disease (DKD) is the major complication of diabetes concomitant with gut dysbiosis and glycometabolic disorder, which are strongly associated with bile acid (BA) metabolism. Yet studies investigating the BA metabolism involving in DKD pathogenesis are limited. This study aimed to explore the metabolomic profiling of BAs in DKD and analyze its association with DKD progression.

Methods

An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established to quantify BAs in the plasma, fecal and urine samples of patients with DKD or T2DM and healthy individuals (n = 30 for each group). The key BAs associated with DKD were identified by orthogonal partial least-squares discriminant analysis (OPLS-DA) and receiver-opera

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC11464666 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature