Efficient conversion of hemicellulose into high-value product and electric power by enzyme-engineered bacterial consortia.

ABSTRACT: As an abundant agricultural and forestry biomass resource, hemicelluloses are hard to be effectively degraded and utilized by microorganisms due to the constraints of membrane and metabolic regulations. Herein, we report a synthetic extracellular metabolic pathway with hemicellulose-degrading-enzymes controllably displayed on Escherichia coli surface as engineered bacterial consortia members for efficient utilization of xylan, the most abundant component in hemicellulose. Further, we develop a hemicellulose/O₂ microbial fuel cell (MFC) configuring of enzyme-engineered bacterial consortia based bioanode and bacterial-displayed laccase based biocathode. The optimized MFC exhibited an open-circuit voltage of 0.71 V and a maximum power density (P_max) of 174.33 ± 4.56 µW c

SUBMITTER: Liang B 

PROVIDER: S-EPMC11464693 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature