Biochemical rationale for transfusion of high titre COVID-19 convalescent plasma.

ABSTRACT: We aimed to model binding of donor antibodies to virus that infects COVID-19 patients following transfusion of convalescent plasma (CCP). An immunosorbent assay was developed to determine apparent affinity (K_{d, app}). Antibody binding to virus was modelled using antibody concentration and estimations of viral load. Assay and model were validated using reference antibodies and clinical data of monoclonal antibody therapy. A single K_{d, app} or two resolvable K_{d, app} were found for IgG in 11% or 89% of CCP donations, respectively. For IgA this was 50%-50%. Median IgG K_{d, app} was 0.8nM and 3.6nM for IgA, ranging from 0.1-14.7nM and 0.2-156.0nM respectively. The median concentration of IgG was 44.0nM (range 8.4-269.0nM) and significantly higher than IgA

SUBMITTER: Verbrugghe C 

PROVIDER: S-EPMC11464705 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature