Quantitative phase imaging verification in large field-of-view lensless holographic microscopy via two-photon 3D printing.

ABSTRACT: Large field-of-view (FOV) microscopic imaging (over 100 mm²) with high lateral resolution (1-2 μm) plays a pivotal role in biomedicine and biophotonics, especially within the label-free regime. Lensless digital holographic microscopy (LDHM) is promising in this context but ensuring accurate quantitative phase imaging (QPI) in large FOV LDHM is challenging. While phantoms, 3D printed by two-photon polymerization (TPP), have facilitated testing small FOV lens-based QPI systems, an equivalent evaluation for lensless techniques remains elusive, compounded by issues such as twin-image and beam distortions, particularly towards the detector's edges. Here, we propose an application of TPP over large area to examine phase consistency in LDHM. Our research involves fabricating widefield

SUBMITTER: Wdowiak E 

PROVIDER: S-EPMC11464779 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature