Multicellular ovarian cancer spheroids: novel 3D model to mimic tumour complexity.

ABSTRACT: In vitro, spheroid models have become well established in cancer research because they can better mimic certain characteristics of in vivo tumours. However, interaction with the tumour microenvironment, such as cancer-associated fibroblasts, plays a key role in tumour progression. We initially focused on the interaction of tumour cells with fibroblasts. To model this interaction, we developed a spheroid model of ovarian cancer and fibroblasts. To this end, ovarian cancer cell lines and ex vivo primary cells were simultaneously and sequentially seeded with fibroblasts in a scaffold-free system at different ratios and subsequently characterized with respect to changes in morphology, proliferation, and viability. We demonstrated that co-cultures are able to form by far more compact spheroids,

SUBMITTER: Florkemeier I 

PROVIDER: S-EPMC11464915 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature