Spatiotemporal dynamics of giant viruses within a deep freshwater lake reveal a distinct dark-water community.

ABSTRACT: Giant viruses (GVs) significantly regulate the ecological dynamics of diverse ecosystems. Although metagenomics has expanded our understanding of their diversity and ecological roles played in marine environments, little is known about GVs of freshwater ecosystems. Most previous studies have employed short-read sequencing and therefore resulted in fragmented genomes, hampering accurate assessment of genetic diversity. We sought to bridge this knowledge gap and overcome previous technical limitations. We subjected spatiotemporal (2 depths × 12 months) samples from Lake Biwa to metagenome-assembled genome reconstruction enhanced by long-read metagenomics. This yielded 293 GV metagenome-assembled genomes. Of these, 285 included previously unknown species in five orders of nucleocytoviruses an

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11465185 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature