Manufacturing of the highly active thermophile PETases PHL7 and PHL7mut3 using Escherichia coli.

ABSTRACT:

Background

The global plastic waste crisis requires combined recycling strategies. One approach, enzymatic degradation of PET waste into monomers, followed by re-polymerization, offers a circular economy solution. However, challenges remain in producing sufficient amounts of highly active PET-degrading enzymes without costly downstream processes.

Results

Using the growth-decoupled enGenes e^X-press V2 E. coli strain, pH, induction strength and feed rate were varied in a factorial-based optimization approach, to find the best-suited production conditions for the PHL7 enzyme. This led to a 40% increase in activity of the fermentation supernatant. Optimization of the expression construct resulted in a further 4-fold activity gain. Finally, the identified improvements

SUBMITTER: Fohler L 

PROVIDER: S-EPMC11465579 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature