Dense Collagen I as a Biomimetic Material to Track Matrix Remodelling in Renal Carcinomas.

ABSTRACT: Aims: Renal tissue is a dynamic biophysical microenvironment, regulating healthy function and influencing tumor development. Matrix remodelling is an iterative process and aberrant tissue repair is prominent in kidney fibrosis and cancer. Biomimetic 3D models recapitulating the collagen composition and mechanical fidelity of native renal tissue were developed to investigate cell-matrix interactions in renal carcinomas. Methods: Collagen I and laminin hydrogels were engineered with renal cancer cells (ACHN and 786-O), which underwent plastic compression to generate dense matrices. Mechanical properties were determined using shear rheology and qPCR determined the gene expression of matrix markers. Results: The shear modulus and phase angle of acellular dense collagen I g

SUBMITTER: Upadhyay A 

PROVIDER: S-EPMC11465592 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature