Proteomic analysis of plasma exosomes in patients with metastatic colorectal cancer.

ABSTRACT:

Background

The diagnosis and treatment of colorectal cancer (CRC), especially metastatic colorectal cancer (mCRC), is a major priority and research challenge. We screened for expression differences in the plasma exosomal proteomes of patients with mCRC, those with CRC, and healthy controls (HCs) to discover potential biomarkers for mCRC.

Methods

Plasma samples from five patients with mCRC, five patients with CRC, and five HCs were collected and processed to isolate exosomes by ultracentrifugation. Exosomal protein concentrations were determined using the BCA kit, and liquid chromatography-mass spectrometry was utilized to identify and analyze the proteins.

Results

From the exosomes isolated from plasma samples, a total of 994 quantifiable proteins were detected, inclu

SUBMITTER: Zhong Z 

PROVIDER: S-EPMC11465920 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature