In Situ Structural Characterization of Cardiomyocyte Microenvironment by Multimodal STED Microscopy.

ABSTRACT: Within the myocardium, cardiomyocytes reside in a complex and dynamic extracellular matrix (ECM) consisting of a basement membrane (BM) and interstitial matrix. The interactions between cardiomyocytes and the myocardial ECM play a critical role in maintaining cardiac geometry and function throughout cardiac development and in adult hearts. Understanding how the structural changes of the myocardial ECM affect cardiomyocyte function requires knowledge of pericellular structures. These structures are of a size beyond the resolution of conventional optical microscopy. Here, we demonstrated multi-scale and multi-aspect characterization of the cardiomyocyte microenvironment in myocardial tissue sections using multimodal stimulated emission depletion (STED) microscopy. Second harmonic generation

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC11466047 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature