A novel gene silencing strategy based on tobacco rattle virus in Hibiscus mutabilis.

ABSTRACT:

Background

Hibiscus mutabilis L. is a popular regional characteristic plant in China, cultivated for its attractive flower colors, extended bloom time, and medicinal properties. To enhance molecular breeding and gene function studies, we conducted transcriptome analysis and identified valuable genes in previous research. Nonetheless, the current inefficient and labor-intensive transformation techniques have hindered their applications. Virus-induced gene silencing (VIGS) provides a precise and effective strategy for post-transcriptional down-regulation of endogenous gene expression.

Methods

We investigated the performance of tobacco rattle virus (TRV) as a tool for targeting and silencing the gene encoding the protein involved in chloroplast development, cloroplastos

SUBMITTER: Sang S 

PROVIDER: S-EPMC11466215 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature