<i>In vitro</i> vascularization of 3D cell aggregates in microwells with integrated vascular beds.

ABSTRACT: Most human tissues possess vascular networks supplying oxygen and nutrients. Engineering of functional tissue and organ models or equivalents often require the integration of artificial vascular networks. Several approaches, such as organs on chips and three-dimensional (3D) bioprinting, have been pursued to obtain vasculature and vascularized tissues in vitro. This technical feasibility study proposes a new approach for the in vitro vascularization of 3D microtissues. For this, we thermoform arrays of round-bottom microwells into thin non-porous and porous polymer films/membranes and culture vascular beds on them from which endothelial sprouting occurs in a Matrigel-based 3D extra cellular matrix. We present two possible culture configurations for the microwell-integrated va

SUBMITTER: Fois MG 

PROVIDER: S-EPMC11466645 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature