Development of human innate immune responses in a humanized mouse model expressing four human myelopoiesis transgenes.

ABSTRACT:

Background

Dysregulated innate immune responses underlie multiple inflammatory diseases, but clinical translation of preclinical innate immunity research in mice is hampered by the difficulty of studying human inflammatory reactions in an in vivo context. We therefore sought to establish in vivo human inflammatory responses in NSG-QUAD mice that express four human myelopoiesis transgenes to improve engraftment of a human innate immune system.

Methods

We reconstituted NSG-QUAD mice with human hematopoietic stem and progenitor cells (HSPCs), after which we evaluated human myeloid cell development and subsequent human responses to systemic and local lipopolysaccharide (LPS) challenges.

Results

NSG-QUAD mice already displayed engraftment of human monocytes,

SUBMITTER: Stocks H 

PROVIDER: S-EPMC11466769 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature