Release kinetics of growth factors loaded into β-TCP ceramics in an in vitro model.

ABSTRACT:

Introduction

β-TCP ceramics are bone replacement materials that have recently been tested as a drug delivery system that can potentially be applied to endogenous substances like growth factors found in blood platelets to facilitate positive attributes.

Methods

In this work, we used flow chamber loading to load β-TCP dowels with blood suspensions of platelet-rich plasma (PRP), platelet-poor plasma (PPP), or buffy coat (BC) character. PRP and BC platelet counts were adjusted to the same level by dilution. Concentrations of TGF-β1, PDGF-AB, and IGF-1 from dowel-surrounding culture medium were subsequently determined using ELISA over 5 days. The influence of alginate was additionally tested to modify the release.

Results

Concentrations of TGF-β1 and PDGF-AB increased and

SUBMITTER: Waldmann M 

PROVIDER: S-EPMC11466813 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature