Fitness factors impacting survival of a subsurface bacterium in contaminated groundwater.

ABSTRACT: Many factors contribute to the ability of a microbial species to persist when encountering complexly contaminated environments, including time of exposure, the nature and concentration of contaminants, availability of nutritional resources, and possession of a combination of appropriate molecular mechanisms needed for survival. Herein we sought to identify genes that are most important for survival of Gram-negative Enterobacteriaceae in contaminated groundwater environments containing high concentrations of nitrate and metals using the metal-tolerant Oak Ridge Reservation isolate, Pantoea sp. MT58 (MT58). Survival fitness experiments in which a randomly barcoded transposon insertion (RB-TnSeq) library of MT58 was exposed directly to contaminated Oak Ridge Reservation groundwater samples fr

SUBMITTER: Thorgersen MP 

PROVIDER: S-EPMC11467524 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature