Investigation of the cytotoxic effect of current dentine bonding agents on human dental pulp cells.

ABSTRACT:

Background

An ideal aesthetic restorative material should be attached to the tooth tissues by adhesion, have a smooth surface as possible, should not cause toxic reactions in the pulp and discoloration and microleakage. This study aims at comparatively assess the cytotoxicity of current adhesive systems on human dental pulp cells.

Materials and methods

The adequate density of human pulp cells was observed from the ready cell line. The passaging was performed and the 3rd passage cells were selected. Adhesive systems and MTA were used on the cultures. Trypan blue staining was conducted on the cells at the 1st, 2nd, 3rd days and a count of live and dead cells using a light microscope. The dead cells whose membrane integrity was impaired by staining with trypan blue and the viab

SUBMITTER: Koruyucu M 

PROVIDER: S-EPMC11468065 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature