Microphysiological Modeling of Gingival Tissues and Host-Material Interactions Using Gingiva-on-Chip.

ABSTRACT: Gingiva plays a crucial barrier role at the interface of teeth, tooth-supporting structures, microbiome, and external agents. To mimic this complex microenvironment, an in vitro microphysiological platform and biofabricated full-thickness gingival equivalents (gingiva-on-chip) within a vertically stacked microfluidic device is developed. This design allowed long-term and air-liquid interface culture, and host-material interactions under flow conditions. Compared to static cultures, dynamic cultures on-chip enabled the biofabrication of gingival equivalents with stable mucosal matrix, improved epithelial morphogenesis, and barrier features. Additionally, a diseased state with disrupted barrier function representative of gingival/oral mucosal ulcers is modeled. The apical flow feature is uti

SUBMITTER: Muniraj G 

PROVIDER: S-EPMC11468103 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature