Functional siRNA Delivery by Extracellular Vesicle-Liposome Hybrid Nanoparticles.

ABSTRACT: The therapeutic use of RNA interference is limited by the inability of siRNA molecules to reach their site of action, the cytosol of target cells. Lipid nanoparticles, including liposomes, are commonly employed as siRNA carrier systems to overcome this hurdle, although their widespread use remains limited due to a lack of delivery efficiency. More recently, nature's own carriers of RNA, extracellular vesicles (EVs), are increasingly being considered as alternative siRNA delivery vehicles due to their intrinsic properties. However, they are difficult to load with exogenous cargo. Here, EV-liposome hybrid nanoparticles (hybrids) are prepared and evaluated as an alternative delivery system combining properties of both liposomes and EVs. It is shown that hybrids are spherical particles encapsu

SUBMITTER: Evers MJW 

PROVIDER: S-EPMC11468224 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature