Macromolecular Cargo Encapsulation via In Vitro Assembly of Two-Component Protein Nanoparticles.

ABSTRACT: Self-assembling protein nanoparticles are a promising class of materials for targeted drug delivery. Here, the use of a computationally designed, two-component, icosahedral protein nanoparticle is reported to encapsulate multiple macromolecular cargoes via simple and controlled self-assembly in vitro. Single-stranded RNA molecules between 200 and 2500 nucleotides in length are encapsulated and protected from enzymatic degradation for up to a month with length-dependent decay rates. Immunogenicity studies of nanoparticles packaging synthetic polymers carrying a small-molecule TLR7/8 agonist show that co-delivery of antigen and adjuvant results in a more than 20-fold increase in humoral immune responses while minimizing systemic cytokine secretion associated with free adjuvant. Coupled with

SUBMITTER: Herpoldt KL 

PROVIDER: S-EPMC11468305 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature