Protein profile of extracellular vesicles derived from adult Parascaris spp.

ABSTRACT:

Background

Parascaris spp. represent a significant threat to equine health worldwide, particularly in foals. The long-term survival of parasites in the host necessitates persistent modulation of the host immune response. Intercellular communication achieved through the exchange of molecules via extracellular vesicles (EVs) released from the parasite could be a crucial factor in this regard. This study aimed to isolate and characterize EVs released by adult male and female Parascaris worms and conduct a proteomic analysis to identify sex-specific proteins and potential immunomodulatory factors.

Methods

Live adult Parascaris worms were collected, and EVs were isolated from spent culture media using differential ultracentrifugation. Nanoparticle tracking analysis and transmissi

SUBMITTER: Manikantan V 

PROVIDER: S-EPMC11468347 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature