Thyroid-on-a-Chip: An Organoid Platform for In Vitro Assessment of Endocrine Disruption.

ABSTRACT: Thyroid is a glandular tissue in the human body in which the function can be severely affected by endocrine disrupting chemicals (EDCs). Current in vitro assays to test endocrine disruption by chemical compounds are largely based on 2D thyroid cell cultures, which often fail to precisely evaluate the safety of these compounds. New and more advanced 3D cell culture systems are urgently needed to better recapitulate the thyroid follicular architecture and functions and help to improve the predictive power of such assays. Herein, the development of a thyroid organoid-on-a-chip (OoC) device using polymeric membranous carriers is described. Mouse embryonic stem cell derived thyroid follicles are incorporated in a microfluidic chip for a 4 day experiment at a flow rate of 12 µL min^-1

SUBMITTER: Carvalho DJ 

PROVIDER: S-EPMC11468662 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature