MiLoPYP: self-supervised molecular pattern mining and particle localization in situ.

ABSTRACT: Cryo-electron tomography allows the routine visualization of cellular landscapes in three dimensions at nanometer-range resolutions. When combined with single-particle tomography, it is possible to obtain near-atomic resolution structures of frequently occurring macromolecules within their native environment. Two outstanding challenges associated with cryo-electron tomography/single-particle tomography are the automatic identification and localization of proteins, tasks that are hindered by the molecular crowding inside cells, imaging distortions characteristic of cryo-electron tomography tomograms and the sheer size of tomographic datasets. Current methods suffer from low accuracy, demand extensive and time-consuming manual labeling or are limited to the detection of specific types of pro

SUBMITTER: Huang Q 

PROVIDER: S-EPMC11468773 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature