New Bacterial Aryl Sulfotransferases: Effective Tools for Sulfation of Polyphenols.

ABSTRACT: The preparation of pure metabolites of bioactive compounds, particularly (poly)phenols, is essential for the accurate determination of their pharmacological profiles in vivo. Since the extraction of these metabolites from biological material is tedious and impractical, they can be synthesized enzymatically in vitro by bacterial PAPS-independent aryl sulfotransferases (ASTs). However, only a few ASTs have been studied and used for (poly)phenol sulfation. This study introduces new fully characterized recombinant ASTs selected according to their similarity to the previously characterized ASTs. These enzymes, produced in Escherichia coli, were purified, biochemically characterized, and screened for the sulfation of nine flavonoids and two phenolic acids using p-nitr

SUBMITTER: Brodsky K 

PROVIDER: S-EPMC11468790 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature