Microglial priming by IFN-γ involves STAT1-mediated activation of the NLRP3 inflammasome.

ABSTRACT:

Background

Inflammatory and immune responses in the brain that contribute to various neuropsychiatric disorders may begin as microglial "priming". Interferon (IFN)-γ is known to cause microglial priming, but the mechanism is unclear.

Methods

We examined the effects of IFN-γ on gene expression, microglial activation, inflammatory and immune responses and activity of the NLRP3 inflammasome in primary microglia and in the brains of mice.

Results

Our results showed that treating microglial cultures with IFN-γ induced a hedgehog-like morphology and upregulated markers of microglial activation (CD86, CD11b) and pro-inflammatory molecules (IL-1β, IL-6, TNF-α, iNOS), while downregulating markers of microglial homeostasis (CX3CR1, CD200R1), anti-inflammatory molecules (MCR1, A

SUBMITTER: He H 

PROVIDER: S-EPMC11468839 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature