Design and Development of Extracellular Matrix Protein-Based Microcapsules as Tools for Bacteria Investigation.

ABSTRACT: The extracellular matrix (ECM) plays an immense role in the homeostasis of tissues and organs, can function as a barrier for infectious agents, but is also exploited by pathogens during infection. Therefore, the development of well-defined 3D ECM models in the form of microcapsules to elucidate the interactions between ECM components and pathogens in confinement and study disease infectivity is important, albeit challenging. Current limitations are mainly attributed to the lack of biocompatible methods for the production of protein-based microcapsules. Herein, hollow ECM-based microcapsules from laminin-111 or laminin-111/collagen IV are generated to investigate the behavior of organisms within confined 3D extracellular matrices. Microcapsules are created using water-in-oil emulsion drople

SUBMITTER: Pashapour S 

PROVIDER: S-EPMC11468930 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature