Microfluidically Aligned Collagen to Maintain the Phenotype of Tenocytes In Vitro.

ABSTRACT: Tendon is a highly organized tissue that transmits forces between muscle and bone. The architecture of the extracellular matrix of tendon, predominantly from collagen type I, is important for maintaining tenocyte phenotype and function. Therefore, in repair and regeneration of damaged and diseased tendon tissue, it is crucial to restore the aligned arrangement of the collagen type I fibers of the original matrix. To this end, a novel, user-friendly microfluidic piggyback platform is developed allowing the controlled patterned formation and alignment of collagen fibers simply on the bottom of culture dishes. Rat tenocytes cultured on the micropatterns of aligned fibrous collagen exhibit a more elongated morphology. The cells also show an increased expression of tenogenic markers at the gene

SUBMITTER: Giacomini F 

PROVIDER: S-EPMC11468977 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature