Nanofiber Topographies Enhance Platelet-Fibrinogen Scaffold Interactions.

ABSTRACT: The initial contact with blood and its components, including plasma proteins and platelets, directs the body's response to foreign materials. Natural scaffolds of extracellular matrix or fibrin contain fibrils with nanoscale dimensions, but how platelets specifically respond to the topography and architecture of fibrous materials is still incompletely understood. Here, planar and nanofiber scaffolds are fabricated from native fibrinogen to characterize the morphology of adherent platelets and activation markers for phosphatidylserine exposure and α-granule secretion by confocal fluorescence microscopy and scanning electron microscopy. Different fibrinogen topographies equally support the spreading and α-granule secretion of washed platelets. In contrast, preincubation of the scaffolds with

SUBMITTER: Kenny M 

PROVIDER: S-EPMC11469041 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature