Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor.

ABSTRACT: Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell signaling observed in vivo and remodel complex native E

SUBMITTER: Rafaeva M 

PROVIDER: S-EPMC11469127 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature