Single-Molecule Binding Assay Using Nanopores and Dimeric NP Conjugates.

ABSTRACT: The ability to measure biomarkers, both specifically and selectively at the single-molecule level in biological fluids, has the potential to transform the diagnosis, monitoring, and therapeutic intervention of diseases. The use of nanopores has been gaining prominence in this area, not only for sequencing but more recently in screening applications. The selectivity of nanopore sensing can be substantially improved with the use of labels, but substantial challenges remain, especially when trying to differentiate between bound from unbound targets. Here highly sensitive and selective molecular probes made from nanoparticles (NPs) that self-assemble and dimerize upon binding to a biological target are designed. It is shown that both single and paired NPs can be successfully resolved and detec

SUBMITTER: Ren R 

PROVIDER: S-EPMC11469134 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature