Fetuin-B Interacts With Insulin Receptor-β and Promotes Insulin Resistance in Retina Cells.

ABSTRACT:

Purpose

The purpose of this study was to investigate the correlation between insulin and Fetuin-B (FETUB) and the influence of FETUB on insulin signaling pathway in diabetic retinopathy (DR).

Methods

Enzyme-linked immunosorbent assay (ELISA) was used to analyze FETUB and insulin levels in the serum and aqueous fluid of patients with DR and healthy controls. Quantitative PCR (q-PCR), Western blotting, and ELISA were used to examine FETUB expression in ARPE-19, BV2, and Müller cells under insulin stimulation. Co-immunoprecipitation was used to investigate the interaction of FETUB with insulin receptor-β (IRβ). Insulin resistance (IR)-BV2 and IR-Müller cells were treated with FETUB recombinant protein or FETUB short hairpin RNA (shRNA) to explore the influence of FETUB on insul

SUBMITTER: Zhang W 

PROVIDER: S-EPMC11469143 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature