Assembly of Multi-Spheroid Cellular Architectures by Programmable Droplet Merging.

ABSTRACT: Artificial multicellular systems are gaining importance in the field of tissue engineering and regenerative medicine. Reconstruction of complex tissue architectures in vitro is nevertheless challenging, and methods permitting controllable and high-throughput fabrication of complex multicellular architectures are needed. Here, a facile and high-throughput method is developed based on a tunable droplet-fusion technique, allowing programmed assembly of multiple cell spheroids into complex multicellular architectures. The droplet-fusion technique allows for construction of various multicellular architectures (double-spheroids, multi-spheroids, hetero-spheroids) in a miniaturized high-density array format. As an example of application, the propagation of Wnt signaling is investigated within het

SUBMITTER: Cui H 

PROVIDER: S-EPMC11469186 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature