Growth Media Conditions Influence the Secretion Route and Release Levels of Engineered Extracellular Vesicles.

ABSTRACT: Extracellular vesicles (EVs) are nanosized cell-derived vesicles produced by all cells, which provide a route of intercellular communication by transmitting biological cargo. While EVs offer promise as therapeutic agents, the molecular mechanisms of EV biogenesis are not yet fully elucidated, in part due to the concurrence of numerous interwoven pathways which give rise to heterogenous EV populations in vitro. The equilibrium between the EV-producing pathways is heavily influenced by factors in the extracellular environment, in such a way that can be taken advantage of to boost production of engineered EVs. In this study, a quantifiable EV-engineering approach is used to investigate how different cell media conditions alter EV production. The presence of serum, exogenous EVs, and other sig

SUBMITTER: Bost JP 

PROVIDER: S-EPMC11469210 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature