Production of Multiple Cell-Laden Microtissue Spheroids with a Biomimetic Hepatic-Lobule-Like Structure.

ABSTRACT: The construction of an in vitro 3D cellular model to mimic the human liver is highly desired for drug discovery and clinical applications, such as patient-specific treatment and cell-based therapy in regenerative medicine. However, current bioprinting strategies are limited in their ability to generate multiple cell-laden microtissues with biomimetic structures. This study presents a method for producing hepatic-lobule-like microtissue spheroids using a bioprinting system incorporating a precursor cartridge and microfluidic emulsification system. The multiple cell-laden microtissue spheroids can be successfully generated at a speed of approximately 45 spheroids min^-1 and with a uniform diameter. Hepatic and endothelial cells are patterned in a microtissue spheroid with the biomi

SUBMITTER: Hong G 

PROVIDER: S-EPMC11469225 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature