A Temperature-Based Easy-Separable (TempEasy) 3D Hydrogel Coculture System.

ABSTRACT: Interactions between different cell types are crucial for their behavior in tissues, but are rarely considered in 3D in vitro cell culture experiments. One reason is that such coculture experiments are sometimes difficult to perform in 3D or require specialized equipment or know-how. Here, a new 3D cell coculture system is introduced, TempEasy, which is readily applied in any cell culture lab. The matrix material is based on polyisocyanide hydrogels, which closely resemble the mechanical characteristics of the natural extracellular matrix. Gels with different gelation temperatures, seeded with different cells, are placed on top of each other to form an indirect coculture. Cooling reverses gelation, allowing cell harvesting from each layer separately, which benefits downstream analysis. To

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC11469334 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature