Seq2scFv: a toolkit for the comprehensive analysis of display libraries from long-read sequencing platforms.

ABSTRACT: Antibodies have emerged as the leading class of biotherapeutics, yet traditional screening methods face significant time and resource challenges in identifying lead candidates. Integrating high-throughput sequencing with computational approaches marks a pivotal advancement in antibody discovery, expanding the antibody space to explore. In this context, a major breakthrough has been the full-length sequencing of single-chain variable fragments (scFvs) used in in vitro display libraries. However, few tools address the task of annotating the paired heavy and light chain variable domains (VH and VL), which is the primary advantage of full-scFv sequencing. To address this methodological gap, we introduce Seq2scFv, a novel open-source toolkit designed for analyzing in vitro display

SUBMITTER: Bachmann Salvy M 

PROVIDER: S-EPMC11469439 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature